At a glance: The CLAHRC NWC Health Inequalities Assessment Toolkit (2017):Coordinators: Popay, J. Porroche-Escudero, A., Sadler, G. and Simpson, S. Lancaster: The National Institute of Health Research Collaboration for Leadership in Applied Health Research and Care North West Coast

This card is an abridged version of the full toolkit for busy professionals so that they can quickly identify and understand the information they need. Visit the website here: www.hiat.org.u

Posttranslational modifications of GLUT4 affect its subcellular localization and translocation

The facilitative glucose transporter type 4 (GLUT4) is expressed in adipose and muscle and plays a vital role in whole body glucose homeostasis. In the absence of insulin, only ~1% of cellular GLUT4 is present at the plasma membrane, with the vast majority localizing to intracellular organelles. GLUT4 is retained intracellularly by continuous trafficking through two inter-related cycles. GLUT4 passes through recycling endosomes, the trans Golgi network and an insulin-sensitive intracellular compartment, termed GLUT4-storage vesicles or GSVs. It is from GSVs that GLUT4 is mobilized to the cell surface in response to insulin, where it increases the rate of glucose uptake into the cell. As with many physiological responses to external stimuli, this regulated trafficking event involves multiple posttranslational modifications. This review outlines the roles of posttranslational modifications of GLUT4 on its function and insulin-regulated trafficking

Suppressed radio emission in supercluster galaxies: enhanced ram pressure in merging clusters?

The environmental influence on the 1.4 GHz continuum radio emission of galaxies is analyzed in a 600 deg2 region of the local Universe containing the Shapley Supercluster (SSC). Galaxies in the FLASH and 6dFGS redshift surveys are cross-identified with NVSS radio sources, selected in a subsample doubly complete in volume and luminosity. Environmental effects are studied through a smoothed density field (normalized with random catalogs with the same survey edges and redshift selection function) and the distance to the nearest cluster (R/r200, where r200 is the virial radius, whose relation to the aperture velocity dispersion is quantified). The fraction of high radio loudness (R_K=L_radio/L_K) galaxies in the 10 Mpc Abell 3558 cluster complex at the core of the SSC (SSC-CR) is half as large than elsewhere. In the SSC-CR, R_K is anti-correlated with the density of the large-scale environment and correlated with R/r200: central brightest cluster galaxies (BCGs) in the SSC-CR are 10x less radio-loud than BCGs elsewhere, with signs of suppressed radio loudness in the SSC-CR also present beyond the BCGs, out to at least 0.3 r200. This correlation is nearly as strong as the tight correlation of L_K with R/r200 (K-luminosity segregation), inside the SSC-CR. The suppression of radio loudness in SSC-CR BCGs can be attributed to cluster-cluster mergers that destroy the cool core and thus the supply of gas to the central AGN. We analytically demonstrate that the low radio loudness of non-BCG galaxies within SSC-CR clusters cannot be explained by direct major galaxy mergers or rapid galaxy flyby collisions, but by the loss of gas supply through the enhanced ram pressure felt when these galaxies cross the shock front between the 2 merging clusters and are later subjected to the stronger wind from the 2nd cluster.Comment: Version consolidated with Erratum A&A 499, 4

A cross-country survey of attitudes toward childbirth technologies and interventions among university students

Problem & Aim: Cultural beliefs that equate birth technology with progress, safety and convenience contribute to widespread acceptance of childbirth technology and interventions. Little is known about attitudes towards childbirth technology and interventions among the next generation of maternity care users and whether attitudes vary by country, age, gender, childbirth fear, and other factors. Methods: Data were collected via online survey in eight countries. Students who had never had children, and who planned to have at least one child were eligible to participate. Findings: The majority of participants (n= 4569) were women (79.3%), and the median age was 22 years. More than half of students agreed that birth technology makes birth easier (55.8 %), protects babies from harm (49.1 %) and that women have a right to choose a medically non-indicated cesarean (50.8 %).Respondents who had greater acceptance of childbirth technology and interventions were from countries with higher national caesarean birth rates, reported higher levels of childbirth fear, and were more likely to report that visual media or school-based education shaped their attitudes toward birth. Positive attitudes toward childbirth technology and interventions were also associated with less confidence in knowledge of birth, and more common among younger and male respondents. Discussion/Conclusion: Educational strategies to teach university students about pregnancy and birth in ways that does not frighten them and promotes critical reflection about childbirth technology are needed. This is especially true in countries with high rates of interventions that reciprocally shape culture norms, attitudes, and expectations

Nuclear–Cytoplasmic Shuttling of the Focal Contact Protein, Zyxin: A Potential Mechanism for Communication between Sites of Cell Adhesion and the Nucleus

Integrin-dependent cell adhesion to specific extracellular matrix molecules has been demonstrated to trigger dramatic changes in gene expression that can affect cell fate. However, little is understood about the molecular mechanism by which events at sites of cell– substratum adhesion are communicated to the cell interior to regulate the transcriptional apparatus. By analogy to classical mechanisms of cell surface receptor function, it seems likely that some components of the integrin-activated signal transduction machinery will be colocalized with cell adhesion molecules. Zyxin is a low abundance phosphoprotein that accumulates with integrins at sites of cell–substratum attachment. Here we show that zyxin exhibits a functional nuclear export signal that is required to keep zyxin concentrated in the cytoplasm and is sufficient to direct nuclear proteins to the cytosol. Furthermore, we demonstrate that native zyxin shuttles between the nucleus and sites of cell adhesion in fibroblasts and is thus an excellent candidate for relaying information between these two compartments